The role of environmental factors in regulating chondrogenesis of mesenchymal stem cells – Implications for cell based cartilage repair therapiesThursday, March 25th, 2010
Thursday March 25, 2010 4:00 – 5:00 p.m.
ME-EM Building, Room 112
University of Dublin, Trinity College
Articular cartilage has a limited capacity for repair. Cell based therapies such asAutologous Chondrocyte Implantation (ACT) can be used to treat cartilage defects, butthere is no conclusive evidence that conventional AC! is more effective thanestablished techniques such as microfracture. This has lead to increased interest innovel tissue engineering strategies and alternatives to chondrocytes such asmesenchymal stem cells (MSC5) for cell-based cartilage repair therapies. Central tothe success of any cell-based therapy is a fundamental understanding of how the localmicroenvironment influences cell phenotype and subsequent matrix synthesis andorganisation. This seminar will first review how our lab is using in vitro models tosystematically investigate how MSCs respond to their biophysical and biochemicalenvironment. Key aspects of the in vivo joint environment, such as oxygen tensionand dynamic compression, will be considered. The seminar will then outline how theinformation provided by such experiments can be used to develop models of cellbehaviour, which can be combined with tools such as the finite element method todevelop predictive tools that can potentially be used to improve outcomes in tissueengineering and regenerative medicine.