Tag: orexin

Lilly’s journey from pre-health to medical lab science and a rewarding career beyond

Shadowing physicians at Dickinson County Hospital was a favorite past-time for Lilly Van Loon ’22. She experienced the hospital: the ER, pediatrics, physical therapy, social work. A career in medicine appealed to Lilly. The pre-health program at Michigan Tech was her choice to begin a journey to medical school. However, she soon realized she did not enjoy the pre-med journey. Maybe it was not the right destination for her.

Image of Michigan Tech Medical Lab Science student Lilly Van Loon
Michigan Tech Medical Lab Science student Lilly Van Loon

But a visit from Karyn Fay, former program director of medical lab science, to her classroom was the redirect she needed. She took the next exit and headed straight to medical lab science (MLS). She loved the analytical nature of MLS. It had process, order and fine detail to master. As she has found, “When you get a sample, you have to problem-solve. You get to help with the diagnosis of the patient. You work independently but you get to collaborate with others.” Lilly enjoyed learning about the different panels, gaining expertise. She knew she was headed in the right direction.

Undergraduate research is a key factor in choosing Michigan Tech

Working in research as an undergraduate was an important factor in selecting Michigan Tech in 2018. She knew she wanted to take part in research. Research came up during an early conversation with an advisor in biological science. Experiential learning from research would increase her knowledge level and round out her resume. She mastered skills like pipetting in the lab. She gained a deeper understanding of different tests and techniques. The repetition gave her confidence. The lab has been an important part of her journey.

Investigating the impact of high salt diets

Lilly’s lab in the Kinesiology and Integrative Physiology department focuses on “The Role of Orexin A in Salt Sensitive Hypertension”. Lilly says “Hypertension is a major risk factor of cardiovascular disease, which is the number one cause of death in the US.  There are many mechanisms that contribute to hypertension, and hyperactivity of orexin signaling is one of them.  Our goal is to see if a high salt diet increases activity of peripheral orexin in Dahl-Salt Sensitive rats. Also, if there is a difference in the expression of orexin between male and female rats. This is super interesting because this condition is so common in the US, and I am excited to understand more about the physiology.”

“Lilly joined our lab in 2020 and learned molecular techniques. She also did animal study including taking care of rats and measuring blood pressure by using a noninvasive tail cuff method. Blood pressure was measured once a week and Lilly seldom missed the measurements,” says Dr. Zhiying (Jenny) Shan, associate professor, kinesiology and integrative physiology. “In our lab we never had blood pressure data of Dahl salt sensitive female rats and Lilly helped us collect this data which fulfills our research. Besides animal work, Lilly dedicated a lot of her time in molecular-based research and worked hard from last summer till now. The PCR and Western blot data that she collected was useful for our future study and will be present in our future publications.”

Image of Western blot analysis of a Dahl-Salt kidney
Western blot analysis of a Dahl-Salt kidney

Lilly sees the benefits of research

Lilly knows the benefits of research for society. She says, “We can identify the limits and strive to learn more…. Without that curiosity and research, our society would remain stagnant and would miss out on a lot of amazing innovations.”

Personally, her research helped her manage time. She loved the research, but it was time consuming. Lilly estimates she committed 10 hours per week as a lab assistant in her sophomore and junior years, and increased it to 15-20 hours per week as a senior. She admits, “You forget this is an extracurricular thing. There’s so much to consider. Balancing research and school work, your mental and physical health, and social life. Thanks to my research I’ve been able to grow my skills in managing time.”

Dr Jenny agrees. “I think Lilly is also a self-disciplined person. Besides research, she has a lot of coursework and a job, but she can handle multiple tasks very well which definitely is due to her discipline and initiative. In addition, Lilly learned things fast, so I really like to work with her, and this is another reason that makes her successful in our lab.”

Lilly receives Undergraduate Research Internship Program grant at Michigan Tech

Receiving an Undergraduate Research Internship Program grant proved exciting. Awards of up to $1,600 are available to all Tech undergraduates interested in engaging in a research experience in a faculty member’s laboratory.  It took a lot of time, sweat and tears to apply for that grant. But it paid off in the end. Her one word to describe the experience: rewarding.

Award recipients are required to present their research at the annual Undergraduate Research Symposium. She accomplished this in March on Friday of Preview Day Weekend with her poster “The Role of Orexin A in Salt Sensitive Hypertension”.

Image of Michigan Tech students presenting research poster
Lilly Van Loon (left) presents “The Role of Orexin A in Salt Sensitive Hypertension” at The Pavlis Honors College’s 2022 Undergraduate Research Symposium, along with Sophia Bancker (right).

Lilly finds community at Michigan Tech

Lilly found the MLS program to be a tight-knit community where students helped each other. The student chapter of the Society of Medical Lab Scientists (SMLS) was a big part of the community. Lilly was responsible for public relations for SMLS. “SMLS has given me so much. I met some of my best friends through it. They will be in my life forever. You do study groups. It’s a great opportunity as an underclassman. You get advice from upperclassmen. As an upperclassman I get to return the favor. They are a great support system. We help each other with registration and studying, getting over the stress of school. We do the blood drive too.”

In looking back at her time at Michigan Tech, she says “This is a place I think you can thrive. I see all my friends, even from different majors, and many are thriving. I don’t think everyone can make it here, but big props to you if you can make it here.” She loves the community at Michigan Tech. “Everyone here welcomes you with open arms. Everyone is so open and friendly, especially in this major. People I met here in my first week are still some of my best friends.”

What’s next?

For Lilly college was always on the radar. She looked forward to exploring interests in science and preparing for that career in medicine. And now she is closer to the destination, with a practicum at Marshfield Clinic this summer as her next stop. Lilly beams when discussion turns to Marshfield Clinic. “There will be cool tests. Because Marshfield Clinic is so well known and brings in patients from all over, there will be things you don’t see every day. I will have three months in the classroom and then 6 months in the lab. Students that have gone through there have done well on their BOC (certification exams) and I am lucky to be one of those people to be a part of it. They have a 100% passing rate for their students.”

Image of Lily smiling with two thumbs up
Two-thumbs up from Lilly on this test result!

Of course, having great students like Lilly in their practicum keeps that passing rate high. Claire Danielson, medical laboratory science program director and academic advisor says “Lilly is a joy to have in the classroom and lights any room she enters! Her positive attitude and strong laboratory skills are going to make her an excellent Medical Laboratory Scientist. We have no doubt that Lilly will make us proud during her practicum at Marshfield Clinic!”

Once she is BOC certificated Lilly will continue her career as a medical lab scientist. She hopes to find a specialization and become a lead technician. Beyond that, she does not rule out returning to school for a masters. Pathology school to become a pathology assistant or entering public health are other options. Lots of exciting stops ahead on Lilly’s journey!

The impact of high levels of research on an undergraduate student at Michigan Tech

Research helps Sophia Bancker complete her goals

Sophia Bancker wanted lab experience. Along with her medical laboratory science (MLS) major and minors in international Spanish and public health, it was an important step on the journey to graduate school. So when Dr. Zhiying (Jenny) Shan asked for undergraduate students to do research and Sophia saw a fit with her schedule, she did not hesitate to raise her hand. This is not surprising for this ambitious Minnesotan who by her own admission does not like to sit still.

Image of Michigan Tech students presenting research poster
Sophia Bancker(right) presents “The Role of Orexin A in Salt Sensitive Hypertension” at The Pavlis Honors College’s 2022 Undergraduate Research Symposium, along with Lilly Van Loon (left).

Research Focus: Understanding the Impact of a High Salt Diet

She works with Dr. Shan and PhD student Xinqian (Sherry) Chen to better understand the impact of high salt diets on hypertension. “What interests me the most about my research is learning what different indicators of hypertension look like and how we test the different body organs to look for identification of Orexin A,” Sophia says. In the lab, she observes the adrenal glands, kidneys, heart, pancreas, and livers, isolates the RNA, and then runs diagnostic tests like PCR and western blot to see if Orexin is present or not, and at what level.

She sees first-hand how high salt diets affect the body and brain. Her work in the lab supports understanding of how the presence of Orexin A is related to high salt diets. She looks at impacts by gender too. Thanks to Sophia’s contributions, we know adrenal gland orexin receptors may be involved in salt-sensitive hypertension. More in vitro research will be done this summer.

The Benefits of Sophia’s Lab Experience at Michigan Tech

Sophia enjoys the lab experience too, particularly in Dr. Shan’s lab, where it is unique that all the faculty and students are women. She finds it to be a comforting environment, where it is safe to ask questions. “I had been kind of shy, so asking questions here was easy. Knowing how much innovation there is and research that has been and still needs to be done is exciting. And I got to be part of that process. I got to jump right in, working in the lab in the spring of 2020, my second semester of freshman year, starting the research right away. I did not have to wash lab dishes for anyone other than myself!” she says.

Sophia gets deep into the science, including the methodology and findings. “I’ve been able to read scientific articles and present them. It was really hard the first time I did it, but it keeps getting easier. I am gaining more confidence in my public speaking skills through it.” But that’s not all. “And I am getting a lot of practice with pipetting, more than I ever would have otherwise.”

Her contributions have not gone unnoticed. Sherry Chan observes, “Sophia is a self-disciplined and self-motivated individual. She attended the lab meetings, as many as she could, and presented in the journal club. She likes to learn everything from the lab and enjoys the lab work even though it is not related to her project. In addition, any lab work that I gave to her she would finish at her earliest convenience. As a researcher, one of the most important capabilities is to test the scientific hypothesis as soon as possible without procrastinating, and Sophia has this precious ability.”

Dr. Shan adds, “Sophia is a hard-working student with strong curiosity. She is eager to learn new techniques. She has a very busy schedule, but she has been trying her best to attend lab meetings, lab Journal clubs and do presentations in the journal club. Her hard-working and curious nature enables her to learn a lot and make great progress quickly.”

Learning about high-salt diets is eye-opening. But Sophia admits understanding what the day-to-day in a research lab looks like, the importance of asking questions and learning by doing, and how to read and present information from scientific journals has been the most gratifying component of her research.

The influence on her future has been as great as the impact of a high salt diet on your heart and kidney. “My experience changed my career choice. I had thought of medical school, but as I studied for MCAT and thought about the sacrifice (many years of medical school), I decided it wasn’t for me. I have decided to apply to PA (Physician’s Assistant) school instead.” Working as a PA is much more appealing to Sophia as knows she will “be more hands-on with people, connecting with them, and still provide the medical care they need. There’s also more work-life balance.” And she will get to do it sooner.

Her academic advisor Claire Danielson believes Sophia is well prepared for PA school. “Sophia is an extremely driven student in the medical laboratory science (MLS) program. She works hard to achieve her goal of going to PA school. Her valiant effort in our program and in undergraduate research does not go unnoticed. Sophia is an excellent role model for students in the MLS program. We wholeheartedly support her future goals and successes.” 

Another outcome of her research is that she was named first-place winner in The Pavlis Honors College’s 2022 Undergraduate Research Symposium for her work on “The Role of Orexin A in Salt Sensitive Hypertension.” Sophia shares, “it meant a lot to be rewarded for the effort and time doing the research, as well as preparing for the presentation. I took a lot of pride in the fact that I was able to readily relay all that I had learned! Public speaking  has not always been one of my finest skills.”

Image of Michigan Tech student Sophia Bancker enjoying the Keweenaw outdoors
When she’s not in the lab, Sophia enjoys the spectacular views of the Keweenaw.

Sophia’s Advice for Undergraduate Students Wanting to do Research

Michigan Tech is a STEM school with many labs for undergraduate students to do research. So the idea of a first- or second-year student doing research is not unusual here. Sophia’s advice for students looking to get involved in research at Michigan Tech is simple. “Talk to a professor. If you have an interest in a specific area and they cannot accommodate you in their lab, they likely know someone else who may need you in their lab.”

Sophia’s advice does not end there. She encourages students starting out at Michigan Tech to “join a fun club. Even just one. I really enjoyed the ski club. My friends and I started the pickleball club, too. There are about ten of us and we are looking to expand.” 

This summer, Sophia will study abroad in Ecuador and Peru with MedLife.  Working in South America at a mobile clinic focusing on treating Covid-19 and improving general hygiene. She looks forward to a hands-on experience in general medicine and public health, areas she thinks she will be working in many years down the road.

As Sophia looks back on her college experience, she reflects “I wish I did not put so much pressure on myself. Every exam did not have to be an ‘A’. A ‘B’ on your transcript is not the end of the world.” This leads us to conclude pressure and high salt diets are not good; terrific research experiences equals great!