Chemistry Newsblog

Tag: national institutes of health

In Print: Tatyana Karabencheva-Christova Revealing the Catalytic Strategy of FTO

Published
By
Diagram of the FTO enzyme catalysis
The FTO enzyme catalyzes the reaction of hydroxylation of 6-methyladenine (m6A) to 6-hydroxymethyl adenine (hm6-A) and then the reaction of oxidation of hm6-A to 6-formyl adenine (f6A). Mutations of the residues in the second coordination sphere influence the activation barrier of the Hydrogen Atom Transfer (HAT) which is the rate-limiting step.

A team from the Department of Chemistry led by Associate Professor Tatyana Karabencheva-Christova and including Ph.D. student Ann Varghese (first author); former Ph.D. students Sodiq Waheed ’23 (Ph.D. Computational Chemistry, currently a researcher at Lilly Corporate Center, Indianapolis) and Shobhit Chaturvedi ’22 (M.S. Ph.D. Chemistry, currently a postdoctoral researcher at UCLA); and undergraduate students Isabella DiCastri, Ciara LaRouche and Brendan Kaski has published an article in Chem Catalysis.

The article is titled “Revealing the catalytic strategy of FTO.” It provides unique insight into the catalytic mechanism of the fat-mass and obesity-associated protein (FTO), which is an Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase targeted for treating obesity and cancer. The study reveals that the mutant R316Q, which is related to diseases, slows down the O2 activation and hydrogen atom transfer rates.

Professor Christo Christov (Chem) is a collaborator in this study, along with Nicolai Lehnert of the University of Michigan and Deyu Li of the University of Rhode Island.

The study is funded by the National Institutes of Health grant 2R15GM132873-02.

About the Chemistry Department at Michigan Technological University

Chemists at Michigan Technological University help students apply academic concepts to real-world issues and advance research making contributions to health and well-being, environmental protection, responsible use of materials, and climate stabilization. The Chemistry Department offers five undergraduate degrees, an MS and PhD in Chemistry, and an accelerated MS. Supercharge your chemistry skills to meet the demands of a technology-driven society at a flagship public research university powered by science, technology, engineering, and math. Graduate with the theoretical knowledge and practical experience needed to solve real-world problems and succeed in academia, research, and tomorrow’s high-tech business landscape.

Questions? Contact us at chemistry@mtu.edu. Follow all the latest happenings on the Chemistry Blog.

Continue Reading →

New Funding: National Institutes of Health Awards Haiying Liu and Rudy Luck

Published
By
Haiying Liu
Haiying Liu

Haiying Liu has been awarded a supplementary grant of $18,995 from the National Institutes of Health. The grant supplements Liu’s parent grant related to the project titled “Near-infrared Fluorescent Probes for Sensitive Detection of NADH in Live Cells,” bringing the total accumulated funding to $488,495.

Rudy Luck is a co-PI on this potential three-year project.

Rudy Luck
Rudy Luck

This supplemental grant will enable the acquisition of a cell incubator equipped with precise oxygen level control. Controlling oxygen levels offers significant advantages for studying NAD(P)H levels in live cells during drug treatment, including enhanced accuracy, reproducibility and the ability to mimic specific physiological conditions.

The research goals of this project involve the design and development of near-infrared fluorescent probes, facilitating accurate and quantitative analysis of alterations in NAD(P)H concentrations within the mitochondria of live cells, specifically within the glycolysis pathway. This comprehensive analysis will encompass diverse metabolic processes and the variations occurring during mitophagy induced by cell starvation and drug treatment. The overarching objective is to attain a more profound understanding of both physiological and pathological processes.

About the Chemistry Department

Chemists at Michigan Technological University help students apply academic concepts to real-world issues and advance research making contributions to health and well-being, environmental protection, responsible use of materials, and climate stabilization. The Chemistry Department offers five undergraduate degrees and a master’s and PhD in chemistry. Supercharge your chemistry skills to meet the demands of a technology-driven society at a flagship public research university powered by science, technology, engineering, and math. Graduate with the theoretical knowledge and practical experience needed to solve real-world problems and succeed in academia, research, and tomorrow’s high-tech business landscape.

Questions? Contact us at chemistry@mtu.edu.

Continue Reading →